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Flow Cytometry

Flow cytometry is a technology that is used to analyze the physical and chemical characteristics of particles in a fluid as it passes through at least one laser. Cell components are fluorescently labeled and then excited by the laser to emit light at varying wavelengths.

The fluorescence can be measured to determine various properties of single particles, which are usually cells. Up to thousands of particles per second can be analyzed as they pass through the liquid stream. Examples of the properties measured include the particle’s relative granularity, size and fluorescence intensity as well as its internal complexity. An optical-to-electronic coupling system is used to record the way in which the particle emits fluorescence and scatters incident light from the laser.

Flow cytometry is used to perform several procedures including:

  • Cell counting
  • Cell sorting
  • Detection of biomarkers
  • Protein engineering

Flow cytometry has numerous applications in science, including those relevant to healthcare. The technology has been widely used in the diagnosis of health conditions, particularly diseases of the blood such as leukemia, although it is also commonly used in the various different fields of clinical practice as well as in basic research and clinical trials.

Flow cytometry can also be used in the field of protein engineering, to help identify cell surface protein variants.

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